Analyzer · Sparsifire

Analyze how specific documents activate the features of the SAE. Select a dataset, a run and a document to begin.

Dataset

SAE Model

Document

Active Features

1544

MIN ACTIVATION

0.0

Details List

#2416 Feature 2416

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#3064 Surgical Complications and Physiological Stress

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#2232 Medical Case Reports & Cancer Research

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#2520 Feature 2520

#1992 Feature 1992

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#251 Feature 251

#1317 Feature 1317

#1830 Feature 1830

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#1433 Feature 1433

#1719 Feature 1719

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#2171 Molecular and Cellular Biological Processes

#1065 Feature 1065

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#470 Feature 470

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#1924 Feature 1924

#800 Feature 800

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#596 Feature 596

#339 Feature 339

#2438 Feature 2438

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Document Content

Bend and helical twist associated with a symmetric internal loop from 5S ribosomal RNA.
We have used gel electrophoretic mobility measurements to investigate the conformation of the symmetric eubacterial loop E sequence of 5S rRNA (seven nucleotides in each strand). The loop strongly retarded the gel mobility of duplex RNAs containing it. In contrast, only asymmetric A5.An or U5.Un internal loops (n not equal to 5) strongly affected duplex RNA gel mobility. A phasing experiment, in which an A2 bulge and loop E were placed in the same duplex RNA and the number of base pairs between them varied, showed that loop E has a permanent bend and is torsionally stiff. A second phasing experiment substituting loop E for duplex sequences between two A2 bulges measured the helical twist associated with loop E; it is about 30 degrees (+/- 15 degrees) overwound compared to a duplex RNA of the same number of bases. Ribosomal protein L25 specifically recognizes loop E but had little or no effect on the twist of the loop. These results suggest that loop E adopts a specific, roughly helical structure.

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